Control 3 - SRA

SRX25990352: Control 3
1 VELA_DIAGNOSTICS (Sentosa SQ301) run: 17.8M spots, 5.3G bases, 1.9Gb downloads

Design: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 1000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Poly(A) RNA is purified from total RNA(5ug) using poly-T oligo-attached magnetic beads using two rounds of purification. Following purification, the mRNA is fragmented into smallpieces using divalent cations under elevated temperature. Then the cleaved RNA fragments werereverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNASeq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-endlibraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (LC Sceiences,USA) following the vendors recommended protocol.

Submitted by: Jiaxing university

Study: Transcriptomics of the fungi Clonostachys rosea was employed to investigate the effcets of fungi on algal cells

PRJNA1156068 • SRP530917 • All experiments • All runs

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The objective of this study was to examine the effects of the fungi Clonostachys rosea on the growth and photosynthesis of the freshwater microalgae Chlorella sp. during indirect contact co-culture, investigating signaling metabolites related to the interaction of fungi and microalgae.

Sample: Control 3

SAMN43481929 • SRS22564827 • All experiments • All runs

Organism: Clonostachys rosea

Library:

Name: FC_2_R1.fastq

Instrument: Sentosa SQ301

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 17.8M spots, 5.3G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR30567224	17,783,822	5.3G	1.9Gb	2024-09-06

ID:: 35083225

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